Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia.
J Biosci
;
2003 Mar; 28(2): 163-8
Article
in English
| IMSEAR
| ID: sea-111324
ABSTRACT
Infertility affects 15% couples attempting pregnancy and in 40-50% of these cases the male partner has qualitative or quantitative abnormalities of sperm production. Microdeletions in the azoospermia factor (AZF) region on the long arm of the Y chromosome are known to be associated with spermatogenic failure and have been used to define three regions on Yq (AZFa, AZFb and AZFc) which are critical for spermatogenesis and are recurrently deleted in infertile males. Semen analysis was carried out on one hundred and twenty five infertile males with oligozoospermia and azoospermia. Cytogenetic analysis was done for all the cases and in all cytogenetically normal cases (n = 83) microdeletion analysis was carried out on DNA extracted from peripheral blood using PCR. The sequence tagged sites (STS) primers sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc) were used for each case. Eight of the eighty three cases (9.63%) showed deletion of at least one of the STS markers. Correlation of phenotype with microdeletion was done in each case to determine any phenotype association with deletion of particular AZF locus. Based on the present study, the frequency of microdeletion in the Indian population is 9.63%. This study emphasizes the need for PCR analysis for determining genetic aetiology in cases with idiopathic severe testiculopathy.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oligospermia
/
Humans
/
Male
/
Polymerase Chain Reaction
/
Chromosome Deletion
/
Adult
/
Chromosomes, Human, Y
Type of study:
Diagnostic study
/
Qualitative research
/
Screening study
Language:
English
Journal:
J Biosci
Year:
2003
Type:
Article
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