Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.
J Biosci
;
2001 Dec; 26(5): 635-40
Article
in English
| IMSEAR
| ID: sea-111330
ABSTRACT
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Protein Precursors
/
Solubility
/
Recombinant Proteins
/
Blotting, Western
/
Cloning, Molecular
/
Concanavalin A
/
Plant Lectins
/
Fabaceae
Language:
English
Journal:
J Biosci
Year:
2001
Type:
Article
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