Dual culture method to determine the relationship of gut bacteria of sandfly (Phlebotomus argentipes) with promastigotes of Leishmania donovani.
Article
in English
| IMSEAR
| ID: sea-112118
ABSTRACT
A simple dual culture agar plating technique has been developed and evaluated for its efficiency in determining the relationship of gut bacteria of sandfly with Leishmania donovani promastigotes. There are about twenty morphologically distinct bacterial colonies have been isolated from the gut homogenate of Phlebotomus argentipes. In dual culture method, each bacterial isolate was inoculated in one half of the plate and the promastigotes of Leishmania was inculcated in the other half by streaking. After incubation, the type of association was determined based on the presence or absence of promastigotes colonies. The reliability of this method was compared with broth dilution method in 96 well plate.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phlebotomus
/
Bacteria
/
Blood
/
Leishmania donovani
/
Humans
/
Culture Media
/
Agar
/
Gastrointestinal Tract
/
Host-Parasite Interactions
/
Insect Vectors
Type of study:
Evaluation studies
Language:
English
Year:
2008
Type:
Article
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