Isoperoxidases show differing sensitivity to salicylic acid.
J Environ Biol
;
2004 Apr; 25(2): 135-40
Article
in English
| IMSEAR
| ID: sea-113441
ABSTRACT
The effects of salicylic acid (SA) on the activity of total peroxidase and the patterns of isoperoxidases of cultured tobacco cells were investigated. The total peroxidase activity of tobacco cells was inhibited by 70% when the cells were treated with 5 mM SA for one week. The peroxidase activity of tobacco cells is declined by 90% in the presence of 30 mM SA. Moreover, the activity of isoperoxidases C3, A1, and A3 decreased dramatically with increasing SA concentration, while, one of the anodic isoperoxidases, A2, was somewhat resistant to SA treatment. When isoperoxidase C3 was isolated, SA inhibited the activity of purified C3 in a concentration-dependent manner. The IC50 of isoperoxidase C3 was approximately 0.45 mM. However, the inhibition of isoperoxidase C3 activity was removed by the addition of Fe2+ ion. The possible mechanism of inhibition of peroxidase by SA is discussed.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Nicotiana
/
Peroxidase
/
Cell Culture Techniques
/
Salicylic Acid
/
Dose-Response Relationship, Drug
/
Isoenzymes
/
Anti-Infective Agents
Type of study:
Diagnostic study
Language:
English
Journal:
J Environ Biol
Year:
2004
Type:
Article
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