Enzymological aspects of bioconversion of m-dinitrobenzene.
J Environ Biol
;
2002 Jul; 23(3): 289-94
Article
in English
| IMSEAR
| ID: sea-113706
ABSTRACT
Three main enzymes, responsible for bioconversion of 1,3 dinitrobenzene (m-DNB) by Micrococcus colpogenes MCM B 410, were isolated from the sonicated cell mass, grown in presence of m-DNB in a synthetic medium, for 7 days. The soluble proteins were separated by differential precipitation and also separated by native PAGE. Each fraction obtained from both the protocols, was tested for nitro aryl reductase, aryl mono oxygenase and resorcinol 2,3 dioxygenase. The apparent molecular weight of the proteins were nitro aryl reductase (30 kDa), aryl mono oxygenase (110 kDa) and resorcinol 2,3 di oxygenase (65 kDa).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Chemical Precipitation
/
Biotransformation
/
Dinitrobenzenes
/
Micrococcus
/
Molecular Weight
Language:
English
Journal:
J Environ Biol
Year:
2002
Type:
Article
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