Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR).
J Environ Biol
;
2008 Jul; 29(4): 543-6
Article
in English
| IMSEAR
| ID: sea-113918
ABSTRACT
Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only forthe identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio spin the samples collected from the natural environment.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Species Specificity
/
Vibrio
/
Water Microbiology
/
RNA, Ribosomal, 16S
/
RNA, Ribosomal, 23S
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Marine Biology
Type of study:
Diagnostic study
Language:
English
Journal:
J Environ Biol
Year:
2008
Type:
Article
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