Nutrient consumption and production of isoflavones in bioreactor cultures of Pueraria lobata (Willd).
J Environ Biol
;
2007 Apr; 28(2): 321-6
Article
in English
| IMSEAR
| ID: sea-113934
ABSTRACT
This paper reports the successful culture of Pueraria lobata (Willd.) suspension cells in a bioreactor. In vitro culture of this Chinese herb has potential as an alternative production method for industrial applications. Calli of P. lobata obtained from leaf explants were cultured in a 5.0 L bioreactor for two weeks. During this period, the pH of the medium declined from 5.8 to 4.5. By the end of the run about 70% of the sugars and reducing sugars and about 50% of nitrate was consumed. Almost 70% of inorganic phosphate and about 80% of the iron was exhausted. The bioreactor results indicated an isoflavone yield of 328.9 microg/ml, with an increase of about 1.77 fold. The yield of puerarin increased about 2.42 fold and reached 73.4 microg/ml in the bioreactor culture.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phosphorus
/
Plant Leaves
/
Bioreactors
/
Pueraria
/
Carbohydrate Metabolism
/
Quaternary Ammonium Compounds
/
Iron
/
Isoflavones
/
Nitrogen
Language:
English
Journal:
J Environ Biol
Year:
2007
Type:
Article
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