Mutations in the S gene region of hepatitis B virus genotype D in Turkish patients.
J Genet
;
2007 Dec; 86(3): 195-201
Article
in English
| IMSEAR
| ID: sea-114339
ABSTRACT
The S gene region of the hepatitis B virus (HBV) is responsible for the expression of surface antigens and includes the 'a'-determinant region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage, permitting the mutant virus to escape from the immune system. The aim of this study was to search for mutations of the S gene region in different patient groups infected with genotype D variants of HBV, and to analyse the biological significance of these mutations. Moreover, we investigated S gene mutation inductance among family members. Forty HBV-DNA-positive patients were determined among 132 hepatitis B surface antigen (HbsAg) carriers by the first stage of seminested PCR. Genotypes and subtypes were established by sequencing of the amplified S gene regions. Variants were compared with original sequences of these serotypes, and mutations were identified. All variants were designated as genotype D and subtype ayw3. Ten kinds of point mutations were identified within the S region. The highest rates of mutation were found in chronic hepatitis patients and their family members. The amino acid mutations 125 (M -> T) and 127 (T -> P) were found on the first loop of 'a'-determinant. The other consequence was mutation inductance in a family member. We found some mutations in the S gene region known to be stable and observed that some of these mutations affected S gene expression.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Turkey
/
Female
/
Humans
/
Male
/
DNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Hepatitis B virus
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
Country/Region as subject:
Asia
Language:
English
Journal:
J Genet
Year:
2007
Type:
Article
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