Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development.
J Genet
;
2009 Apr; 88(1): 33-9
Article
in English
| IMSEAR
| ID: sea-114423
ABSTRACT
The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 x fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male or female parent. We localized fmf-1 to the LG IL genome segment between the centromere-proximal breakpoint of the chromosome segment duplication Dp(IL)39311 and the centromere. By mapping crossovers with respect to RFLP markers in this region we further localized fmf-1 to an approximately 34-kb-genome segment. Partial sequencing of this segment revealed a point mutation in the gene NCU 09387.1, a homologue of the Schizosaccharomyces pombe ste11+ regulator of sexual development. The fmf-1 mutation did not complement a NCU 09387.1 deletion mutation, and transformation with wild-type NCU 09387.1 complemented fmf-1. S. pombe Ste11 protein (Ste11p) is a transcription factor required for sexual differentiation and for the expression of genes required for mating pheromone signalling in matP and matM cells. If FMF-1 also plays a corresponding role in mating pheromone signalling in Neurospora, then protoperithecia in an fmf-1 x fmf-1+ cross would be unable to either send or receive sexual differentiation signals and thus become arrested in development.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Schizosaccharomyces
/
Transcription Factors
/
Fungal Proteins
/
Gene Expression Regulation, Fungal
/
Genome, Fungal
/
Schizosaccharomyces pombe Proteins
/
Models, Genetic
/
Mutation
/
Neurospora crassa
Type of study:
Prognostic study
Language:
English
Journal:
J Genet
Year:
2009
Type:
Article
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