Detection of Rh antibodies using two low ionic diluents: extension of the incubation time and the number of Rh antibodies detected.
J Postgrad Med
;
2008 Jan-Mar; 54(1): 4-6
Article
in English
| IMSEAR
| ID: sea-115852
ABSTRACT
BACKGROUND:
Low ionic strength solution (LISS) is used to increase the rate of association of antibody to the corresponding antigen during antibody detection tests. A number of LISSs are available on the market.AIMS:
The efficiency of two commercial low ionic diluents, DiaMed ID-CellStab and Inverclyde LISS were assessed using the DiaMed-ID LISS Coombs microtube column system and an incubation time varying from 15 to 35 min. MATERIALS ANDMETHODS:
One-hundred samples containing five Rh antibodies (anti-D, anti-C, anti-E, anti-c and anti-e) were tested against commercial red cells using the two low ionic diluents after 15, 25 and 35 min.RESULTS:
The Inverclyde LISS detected 91, 95 and 96% of the Rh antibodies compared to 78, 79 and 83% for ID-CellStab after 15, 25 and 35 min incubation time, respectively, for both methods.CONCLUSIONS:
The Inverclyde LISS is a more suitable and efficient diluent than ID-CellStab for the detection of Rh antibodies. The sensitivity of Rh antibody detection increased for the both methods as the incubation time increased.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Osmolar Concentration
/
Solutions
/
Time Factors
/
Humans
/
Coombs Test
/
Sensitivity and Specificity
/
Isoantibodies
Type of study:
Diagnostic study
/
Evaluation studies
Language:
English
Journal:
J Postgrad Med
Year:
2008
Type:
Article
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