Immunophenotypic characterisation of peripheral T lymphocytes in pulmonary tuberculosis.
J Postgrad Med
;
2008 Jan-Mar; 54(1): 7-11
Article
in English
| IMSEAR
| ID: sea-116316
ABSTRACT
BACKGROUND:
The cellular immune response plays an important role in determining the outcome of infection and disease in Mycobacterium tuberculosis. Many studies of these disease interactions yield contradictory results.AIM:
This study aims at determining the changes that take place in the subpopulations of T lymphocytes in the blood of patients with pulmonary tuberculosis (TB). SETTINGS ANDDESIGN:
This cross-sectional study was done at King Khalid University Hospital, Riyadh, Saudi Arabia. MATERIALS ANDMETHODS:
Flow cytometry was used to determine the absolute numbers and percentages of T CD3, T CD4, T CD8, T CD19 and natural killer (NK) T cells in 54 patients with active pulmonary TB before the commencement of treatment and in 25 healthy PPD negative volunteers. STATISTICALANALYSIS:
Statistical Package for Social Sciences (version 11.5) was used for analysis.RESULTS:
There were significant differences in the values of CD3, CD4 and NK T cells among the groups. The numbers of CD3 and CD4 cells were lower in subjects than in controls [1091.9 +/- 321.4 vs. 1364.6 +/- 251.2; P < 0.001 and 639.8 +/- 285 vs. 822 +/- 189.9; P < 0.004, respectively] while numbers of NK T cells were much higher in patients than in controls (410.7 +/- 286 vs. 182.3 +/- 140; P < 0.001). The numbers of CD8 cells were not significantly changed with disease (609 +/- 233.5 in subjects and 613.4 +/- 170.3 in controls P = 0.761).CONCLUSION:
There are significant changes in the cellular immune response particularly affecting the CD3, CD4 and NK T cells with the development of pulmonary TB. Therefore, further studies of these changes may have important implications on the development of diagnostic tools, vaccines and treatment modalities.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Saudi Arabia
/
Tuberculosis, Pulmonary
/
Aged, 80 and over
/
Aged
/
Female
/
Humans
/
Male
/
T-Lymphocytes
/
Case-Control Studies
/
Cross-Sectional Studies
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Limits:
Aged80
Country/Region as subject:
Asia
Language:
English
Journal:
J Postgrad Med
Year:
2008
Type:
Article
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