Relative efficiency of polymerase chain reaction and enzyme-linked immunosorbant assay in determination of viral etiology in congenital cataract in infants.
J Postgrad Med
;
2008 Jan-Mar; 54(1): 17-20
Article
in English
| IMSEAR
| ID: sea-116878
ABSTRACT
BACKGROUND:
Perinatal viral infections of fetus are among the leading causes of congenital cataract and identifying the viral etiology is important.OBJECTIVES:
To detect the presence of Rubella virus (RV), herpes simplex virus (HSV) and cytomegalovirus (CMV) in lens aspirate specimens obtained from patients with congenital cataract and relate the results with serology. SETTING ANDDESIGN:
Prospective study carried out in tertiary care hospital. MATERIALS ANDMETHODS:
Fifty lens aspirates from 50 infants with congenital cataract were subjected to HSV, RV isolation and polymerase chain reaction (PCR) for detection of HSV and CMV. Reverse transcription polymerase chain reaction (RT-PCR) was applied for RV detection. Peripheral blood specimens were screened for anti-HSV, RV and CMV antibodies by enzyme-linked immunosorbant assay (ELISA).RESULTS:
Rubella virus was detected in nine (18%) lens aspirates, by nRT-PCR which includes six positive by culture. HSV-2 DNA was detected in nine other lens aspirates, while CMV was not detected by PCR. Serological results did not correlate with the presence of viruses in the lens aspirates. This is the first report of detection of HSV-2 DNA in cases of congenital cataract.CONCLUSIONS:
Cytomegalovirus may not be playing a significant role in causation of congenital cataract. The role of serology in identifying causative viral infection for congenital cataract needs to be re-evaluated.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rubella virus
/
Cataract
/
Female
/
Humans
/
Male
/
Infant, Newborn
/
Enzyme-Linked Immunosorbent Assay
/
Polymerase Chain Reaction
/
Prospective Studies
/
Simplexvirus
Type of study:
Etiology study
/
Observational study
Language:
English
Journal:
J Postgrad Med
Year:
2008
Type:
Article
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