Caspase 1 and caspase 8 in HIV infected patients with and without tuberculosis.
J Postgrad Med
;
2004 Apr-Jun; 50(2): 98-101
Article
in English
| IMSEAR
| ID: sea-116924
ABSTRACT
BACKGROUND:
Caspase 8 is involved in apoptosis mediated by Fas and p55 tumor necrosis factor receptor ligation in HIV infection. Apoptosis is partially mediated by interleukin-1beta-converting enzyme (caspase-1).AIMS:
We determined apoptosis, using caspase-1 and caspase-8, among patients with HIV infection, with and without tuberculosis (TB), those with TB alone and healthy individuals. SETTING ANDDESIGN:
Cross-sectional analysis of caspase-1 and caspase-8 among patients with HIV infection, with and without TB, those with TB alone and healthy individuals. MATERIALS ANDMETHODS:
Nineteen HIV infected patients with TB (HIV+/TB+) and 20 with HIV infection without TB (HIV+/TB-) were studied. Fifteen individuals with TB alone were disease controls (HIV-/TB+) and 20 were healthy controls (HIV-/TB-). Caspases were measured by single-step ELISA using commercially available monoclonal antibodies. STATISTICALANALYSIS:
Two-way ANOVA and Pearson's correlation coefficient.RESULTS:
Mean CD4 counts of HIV+/TB+ were lower than HIV+/TB- (p<0.05). OD value of caspase 1 in HIV+/TB+ was 0.295+0.05, while that in HIV+/TB- it was 0.302+0.18. It was 0.293+0.07 in HIV-/TB+ and in HIV-/TB- the values were 0.287+0.06. OD value of caspase 8 in HIV+/TB+ was 0.307+ 0.07, lower than HIV+/TB- (0.927+0.25). It was 0.008+0.03 in HIV-/TB+ and in HIV-/TB-, 0.074+0.004. Values of caspase 8 in patients with HIV infection (with/without TB) were higher than those with TB alone or healthy individuals (p<0.01). Levels of caspase 8 in HIV+/TB- were higher than patients with HIV+/TB+ (p<0.01).CONCLUSION:
Levels of caspase-1 are not different irrespective of presence or otherwise of TB and HIV infection. Fas-related apoptosis is higher in HIV infection. With concomitant TB, levels of caspase 8 were lower as compared with those without TB.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tuberculosis, Pulmonary
/
Female
/
Humans
/
Male
/
Case-Control Studies
/
HIV Infections
/
Cross-Sectional Studies
/
Apoptosis
/
CD4 Lymphocyte Count
/
Adult
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Language:
English
Journal:
J Postgrad Med
Year:
2004
Type:
Article
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