Viability testing of homograft valves using methyl thiazol tetrazolium assay.
J Postgrad Med
;
1996 Jul-Sep; 42(3): 72-5
Article
in English
| IMSEAR
| ID: sea-117574
ABSTRACT
Viability status before and after treatment with antibiotics was investigated in a total of 104 homograft valves using MTT (3-[4, 5-dimethyl thiazol-2-y1]-2, 5-diphenyl tetrazolium bromide). The valves with warm ischaemic time above 11 hours, were found to be non-viable. Increase in storage time directly decreases cell viability. Methyl thiazol tetrazolium (MTT) assay can be used as a reliable, simple, rapid and economic method for assessing the viability status based on mitochondrial respiration even for homograft valves. Basal media with and without nutrients i.e., DMEM and Hanks BSS showed no difference in viability of the cells.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tetrazolium Salts
/
Thiazoles
/
Time Factors
/
Transplantation, Homologous
/
Female
/
Humans
/
Male
/
Cryopreservation
/
Cell Survival
/
Cause of Death
Type of study:
Controlled clinical trial
Language:
English
Journal:
J Postgrad Med
Year:
1996
Type:
Article
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