Markers for population genetic analysis of human plasmodia species, P. falciparum and P. vivax.
J Vector Borne Dis
;
2003 Sep-Dec; 40(3-4): 78-83
Article
in English
| IMSEAR
| ID: sea-117901
ABSTRACT
Present report deals with the genetic diversity existing among the field isolates of Plasmodium falciparum and P. vivax in India. Isoenzymes and molecular markers were used to analyse field isolates of P. falciparum and P. vivax. High level of length polymorphism was observed in repeat nucleotide sequences of MSP-1, MSP-2 and GLURP in P. falciparum isolates and CSP, GAM-1 and MSP-3 alpha in P. vivax isolates. In study populations a high proportion of isolates (up to 60%) were comprised of more than one genetically distinct parasite type--multiclonal. Presence of identical allelic forms of enzyme and DNA variations in different geographical areas and in different years suggest that isolates belong to a single random mating population of P. vivax and P. falciparum. Observed random combination of alleles in the field isolates suggest the unlinked nature of loci studied. Study supports the feasibility of using molecular markers for the identification of recrudescence in P. falciparum from fresh infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium falciparum
/
Plasmodium vivax
/
Polymorphism, Genetic
/
Genetic Variation
/
Humans
/
Protozoan Proteins
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Malaria, Vivax
/
Malaria, Falciparum
Country/Region as subject:
Asia
Language:
English
Journal:
J Vector Borne Dis
Journal subject:
Parasitology
/
Tropical Medicine
Year:
2003
Type:
Article
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