Brain edema after intracerebral hemorrhage in rats: the role of inflammation.
Neurol India
;
2006 Dec; 54(4): 402-7
Article
in English
| IMSEAR
| ID: sea-120411
ABSTRACT
BACKGROUND:
Intracerebral hemorrhage (ICH) results in secondary brain edema and injury that may lead to death and disability. ICH also causes inflammation. It is unclear whether inflammation contributes to brain edema and neuron injury or functions in repairing the brain tissue.AIMS:
To understand the effect of inflammation in ICH, we have carried out an investigation on the various aspects and the dynamic changes of inflammation. SETTINGS ANDDESIGN:
An ICH model was generated by injecting 50 microl autologous tail artery blood stereotactically into the right caudate nucleus of 30 rats, which were randomly divided into five ICH groups. Similarly, five Sham control groups were generated by inserting the needle to the right caudate nucleus of rats. MATERIALS ANDMETHODS:
Rat behavior was evaluated over the time course (6 h, 24 h, 48 h, 72 h and 7 d) in each group. The rats were then killed by administering an overdose of pentobarbital. Following the euthanasia, the brain water content, neuronal loss, glia proliferation, inflammatory infiltration and brain morphology of the rats were measured. Additionally, the expression of TNF-alpha, IL-6, ICAM-1, VEGF, NF-kappaB, C3 and CR2 was analyzed by immunohistochemistry. STATISTICALANALYSIS:
The data were analyzed by student's t test.RESULTS:
Rat brain water content increased progressively over the time course and reached its peak at 48 h followed ICH. The maximum of inflammatory infiltrate (especially neutrophils) and immunopositive cells of TNF-alpha, IL-6 and NF-kappaB, were at 48 h. The expression of C3 and CR2 reached their peaks at 48-72 h, while the expression ICAM-1 and VEGF were at maximum at 72 h followed ICH.CONCLUSIONS:
The results suggested that the inflammatory cytokines, complement system and VEGF may have a function in the development of the brain edema and neuron injury followed ICH.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Brain Edema
/
Male
/
Complement C3
/
Cerebral Hemorrhage
/
NF-kappa B
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Receptors, Complement 3d
/
Rats, Sprague-Dawley
Type of study:
Prognostic study
Language:
English
Journal:
Neurol India
Year:
2006
Type:
Article
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