In-vitro nitric oxide (NO) production of murine cell line and human monocyte-derived macrophages after cytokine stimulation and activation

ABSTRACT

Nitrite (NO2) and nitrate (NO3) production were determined using J774-murine cell line and human monocyte-derived macrophages after activation of these cells with Interferon gamma (IFN-Y), Lipopolysaccharides (LPS) and Tumor Necrotic Factor a (TNF-alp ) in different concentration and in different combination. Both simple colorimetric assay and chemiluminescence assay were used for comparison. Simple colorimetric assay could detect nitrite produced by J774 cells, only However, with chemiluminescence assay nitrite production by both J774 cells and human monocyte-derived macrophages could be measured. This study was aimed to investigate a possible potent killing mechanism of monocytic cells in infection with intracellular microorganisms, particularly mycobacterium.