Diagnosis of human rotavirus in stool specimens: comparison of different methods.
Article
in English
| IMSEAR
| ID: sea-1275
ABSTRACT
A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rotavirus Infections
/
Bangladesh
/
Female
/
Humans
/
Male
/
Infant, Newborn
/
Latex Fixation Tests
/
Child, Preschool
/
Prevalence
/
Immunoenzyme Techniques
Type of study:
Diagnostic study
/
Prevalence study
Country/Region as subject:
Asia
Language:
English
Year:
2006
Type:
Article
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