The histological examination of livers in normal and streptozotocin-induced diabetic rats received Annona squamosa L. aqueous leaves extract

ABSTRACT

The present study aimed to investigate histological characters of livers in normal and streptozotocin (STZ)-induced diabetic rats after receiving various doses of Annona squamosa L. aqueous leave extract. Diabetes was induced by intraperitoneal injection of STZ (45 mg/kg). After that the diabetic rats were orally administered the vehicle, glibenclamide and leaves extract (125, 250 and 500 mg/kg) for 12 days. Their liver tissues were collected for histological examination. The histological study of liver showed that control diabetic rats possessed many hepatocytes with shrunken nuclei, vacuolated granular cytoplasm, poorly defined cell boundaries and dilated sinusoids. The diabetic rats received the extract revealed some improvements of liver tissue in dose dependent manner in which hepatocytes possessed regular size of nuclei, well defined cell boundaries, reduced vacuoles and granulated characters as well as narrowed sinusoids when compared with those of control diabetic rats. This present data suggests that the aqueous leaves extract of Annona squamosa L. could improve the histological appearances of the livers of diabetic rats.