Impaired Implantation Potential, A dose-Dependent Effect of Preimplantation Exposure to Ammonium Chloride

ABSTRACT

Objectives: To investigate the effects of preimplatation exposure of (F1×F1) strain mouse embryos to different dosages (0.3 VS 0.6mM) of ammonium chloride.Study Method: Total of 267, one-cell stage mouse embryos were randomly allocated to culture in either M16 medium (control), or the other two experimental groups which are M16+0.3 mM ammonium chloride or M16-0.6 mM ammonium chloride. Embryos were left in culture for 3 days before being transferred to 2.5 day pseudopregnant recipients. Embryo morphology was assessed after 1, 2 and 3 day of culture. The number of implantation site, fetuses, moles and any gross abnormality found were noted.Results: There was no significant difference in the number of embryos achieved morula or more advanced stage after three days of culture between the two treatment groups. Implantation rate was significantly higher in the group of embryos exposed to 0.3 mM ammonium chloride compared to those exposed to 0.6 mM ammonium chloride (Fisher Exact test, P