Anticlastogenic activity of plumbago indica using mouse bone marrow: Micronucleus test.

ABSTRACT

Certain human cancers can be prevented by identification of mutagenic agents in the environment and protecting humans from exposure to such agents. The purpose of this study is to evaluate the anticlastogenic activity of Plumbago indica in mouse bone marrow cells. The known clastogen used was mitomycin C at 1.5mg/kg body weight. The Plumbago indica extract is treated with mitomycin C at three different dose levels viz., 0.5, 1 & 2mg/kg at varying time periods. The pretreatment was carried out at 3, 6, & 12 hours, and the animals were sacrificed after 24 hours of the last administered injection. The percent inhibition of micronucleated polychromatic erythrocytes (MNPCEs) was found to be more than 50% at all time points of pretreatment. Plumbago indica was found to be effective at 12 hours before treatment of mitomycin C (−80%). The inhibition of micronuclei in MNPCEs induced by mitomycin C was not effective at simultaneous and post treatment of Plumbago indica.