Effects of mechanical stimulus on mesenchymal stem cells differentiation toward cardiomyocytes.
Article
in English
| IMSEAR
| ID: sea-135046
ABSTRACT
Background:
Mesenchymal stem cells (MSCs) known to be sensitive to mechanical stimulus. This type of stimulus plays a role in cellular differentiation, so that it might affect MSCs differentiation toward cardiomyocytes.Objectives:
Investigate the effect of mechanical stimulus on MSCs differentiation toward cardiomyocytes.Methods:
The adipose tissue-derived MSCs were induced to differentiate with 5-azacytidine, and stimulated by one Hz mechanical stretching up to 8%. After 10 days, the cell’s cardiac markers and cardiogenesis-related genes were detected by immumohistochemistrical staining and reverse transcriptase-polymerase chain reaction, and the cell’s ATPase activity was detected.Results:
The cyclic mechanical stretching enhanced the expression of cardiogenesis-related genes and cardiac markers, and stimulated the activity of Na+-K+-ATPase and Ca2+-ATPase in the MSCs treated with 5-azacytidine. Without 5-azacytidine pre-treatment, cyclic mechanical stretch alone has little effect.Conclusion:
Mechanical stretch combined with 5-azacytidine treatment could accelerate MSCs differentiation toward cardiomyocytes.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS