Efficient and rapid purification of lentil -galactosidase by affinity precipitation with alginate.
Indian J Biochem Biophys
;
2009 Oct; 46(5): 366-370
Article
in English
| IMSEAR
| ID: sea-135218
ABSTRACT
-Galactosidase (-D-galactoside galactohydrolase, EC 3.2.1.22) was purified (26-fold) from the germinating seeds of lentil (Lens culinaris) by affinity precipitation with alginate. The purified enzyme gave a single band corresponding to molecular mass of 40 kDa on SDS-PAGE. The optimum temperature and pH of the enzyme were determined as 40oC and 5.5, respectively. The enzyme was very stable at a temperature range of 4-65oC and at a pH range of 4-7. The values of kinetic constants Km and Vmax using p-nitrophenyl--D-galactopyranoside (PNPG) as substrate were 0.191 mM and 0.73 U, respectively. Results suggest that affinity precipitation is an attractive process for the purification of -galactosidase.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Chemical Precipitation
/
Seeds
/
Temperature
/
Time Factors
/
Alpha-Galactosidase
/
Germination
/
Glucuronic Acid
/
Lens Plant
/
Alginates
/
Hexuronic Acids
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2009
Type:
Article
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