Effect of mercury ion on the stability of the lipid-protein complex of isolated chloroplasts.
Indian J Biochem Biophys
;
2009 Oct; 46(5): 405-408
Article
in English
| IMSEAR
| ID: sea-135225
ABSTRACT
Mercury is known to interact with different parts of living systems causing serious biochemical and physiological disorder. In order to know the effect of mercury (Hg2+) ion on chloroplasts, the cell free organelle are incubated in an isotonic buffer medium in presence of mercury ion. The metal ion is found to induce membrane lipid peroxidation, loss of photosynthetic pigments and degradation of proteins. Such degradation brings about a drastic modification of lipid-protein organization of chloroplasts as reflected from a blue shift of absorption peaks and lowering of chlorophyll-a fluorescence intensity. The detrimental effect of Hg2+ ion has been explained in terms of direct binding with lipid-protein complex of photosynthetic membrane. Such a binding of metal ion exposes the lipid-protein complex for an easier entry and attack of reactive oxygen species (ROS) generated during incubation of chloroplasts in light and dark, thereby resulting in higher disorganization, which is evident from cation- induced changes in absorption and emission characteristics of the organelle.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Photosynthesis
/
Pigments, Biological
/
Plant Proteins
/
Triticum
/
Lipid Peroxidation
/
Chloroplasts
/
Thylakoids
/
Darkness
/
Absorption
/
Lipid Metabolism
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2009
Type:
Article
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