In vitro antioxidant activity of Hedyotis corymbosa (L.) Lam. aerial parts.
Indian J Biochem Biophys
;
2010 Feb; 47(1): 49-52
Article
in English
| IMSEAR
| ID: sea-135243
ABSTRACT
The methanolic extract of the aerial part of Hedyotis corymbosa (L.) Lam. (Rubiaceae) was screened for antioxidant activity using 1,1-diphenyl-2-picryl hydroxyl (DPPH) quenching assay, 2,2’-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) cation decolorization test, ferric reducing power (FRP), scavenging capacity towards hydroxyl ion (OH·) radicals and nitric oxide (NO) radical inhibition activity using established assay procedures. Total phenolics and total flavonoid contents were also determined. The plant yielded 210 mg gallic acid equivalent/100 g phenolic content and 55 mg quercetin equivalent/100 g flavonoid content. The extract exhibited high antiradical activity against DPPH, ABTS, nitric oxide and hydroxyl radicals with EC50 value of 82, 150, 130, and 170 µg/ml, respectively. The FRP increased with increasing concentration of the sample. The antioxidant activity of the extract was comparable with that of the standard butylated hydroxyl toluene (BHT). High correlation between total phenolic/flavonoid contents and scavenging potential of different reactive oxygen species (R2 = 0.785-0.998) indicated the polyphenols as the main antioxidants.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Extracts
/
Free Radical Scavengers
/
Reactive Oxygen Species
/
Rubiaceae
/
Antioxidants
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS