Mutational analyses of K-ras exon 2 in Thailand colorectal cancer tissue samples.

ABSTRACT

Objective: The main purpose of this study was to elucidate the genotype of K-ras gene in Thailand colorectal cancer tissue samples, especially in exon 2, which has never before been reported. Methods: 106 patients’ samples in formalin fixed paraffin embedded tissue blocks were investigated in this study. DNA was extracted and PCR was performed by using primers specific for the K-ras gene at exon 2. Direct sequencing was performed in a Genetic Analyser ABI3130 with specific software. Results: The mutation of K-ras exon 2 gene in Thailand colorectal cancer samples accounted for 37.7% of the total. The most common mutation found in this series was the G"A transition which accounted for 70%. Conclusion: The incidence of K-ras exon 2 mutation in Thailand colorectal cancer samples was remarkedly similar to previous reports.