Comparative study of two screening tests for urinary porphyrins in the diagnosis of porphyrias.
Article
in English
| IMSEAR
| ID: sea-137104
ABSTRACT
Objective:
To evaluate the efficiency of two urinary porphyrins screening tests routine fluorescent detection and semi quantitative spectrophotometric scanning.Methods:
Minimal-level detection was performed by adding standard coproporphyrin of 0, 25, 50, 100, 250 and 500 ตg/L in urine and then screened by the two methods. Urine samples from 39 controls, 7 patients with porphyrias and 20 patients with liver impairment were quantitated for total porphyrins, followed by a comparison of the two qualitative tests.Results:
The fluorescent test detected the minimal porphyrin level at 250 ตg/L whereas spectrophotometric scanning could detect a lower level, at 100 ตg/L. Total control subjects showed negative results from both tests while all 7 patients with porphyrias and 6 out of 20 cases of liver impairment showed definite positive results.Conclusion:
Urinary screening for porphyrins from both tests revealed the same accuracy from this study. Still, the spectrophotometric method which is simpler, more sensitive and easily interpretable seemed more practical as a screening test in general laboratories. Keywords Screening test; Porphyrias; Urinary porphyrins
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Diagnostic study
/
Qualitative research
/
Screening study
Language:
English
Year:
2005
Type:
Article
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