Sperm motility and morphology after preparation using the percoll gradient method compared with the IxaPrep gradient method.

ABSTRACT

In order to compare the Percell gradient and IxaPrep gradient methods, the percentage of progressively motile sperm and the percentage of sperm with normal morphology were examined. Thirty five normal semen sample were collected from the male partners of infertile couples attending the Infertility Clinic. The samples were divided into two fractions of 1 ml each. Both fractions were processed using the Percoll gradient and IxaPrep gradient methods, and sperm motility and normal morphology were evaluated. The percentages of spermatozoa that showed progressive motility and normal morphology from the sperm preparation using the IxaPrep gradient method were significantly higher than those prepared by the Percoll gradient method (77.7 + 12.0 vs 73.7 + 12.3 and 64.4 + 15.1 vs 61.1 + 13.3 respectively ; p<0.05). It was concluded that sperm preparation using IxaPrep gradient method showed better sperm quality with respect to progressive motility and normal morphology than those using the Percoll gradient method, The IxaPrep gradient method can be considered as an alternative to the Percoll gradient for sperm preparation.