The detection of blood group in small amount blood stain by microtechnique.

ABSTRACT

A microtechnique adapted from mixed agglutination method was modified in order to detect blood group in small amount of blood stain (approx 10 microgram). By improving the blood stain solvent and method, the ABO grouping of human blood stain in small amount can be detected correctly almost 100% at the duration of 1 month for blood stain in room temperature and 2 weeks for blood stain under influence of sunlight. The technique was described and show that it is possible to be a routine laboratory examination in forensic medicine.