Rapid microscopic determination of spermatozoa from seminal stain by mean of cellophane tape technique.

ABSTRACT

The authors have worked on a technique using pressure sensitive or sticky tape for determination of spermatozoa from a seminal stain. By simply pressing the scotch tape on the stain or gently touching a cotton swab stain to the sticky surface of the tape spermatozoa from the stain can be transferred to the sticky surface. Nest the sticky surface of the tape is covered with a microscopic slide. Third, one end of the tape is firmly fixcal with an adhesive paper band and set up when the slide is vertically out into a coping jar for staining. During the staining process the lower part of the tape may partially free itself from the slide. The modified haematoxylene eosin staining method is recommended. After staining, press the whole tape firmly on the slide in order to get rid of any small air bubbles underneath. Keep the slide dry and in warm place and examine directly under microscope for determination of spermatozoa. This slide does not necessary need any cover glass on it. Comparing yhis new technique with NH4OH soaking of the stain, this technique shows good reliability.