In silico prediction of exposure amino acid sequences of outer inflammatory protein A of Helicobacter pylori for surface display on Eschierchia coli.
Indian J Hum Genet
;
2012 Jan; 18(1): 83-86
Article
in English
| IMSEAR
| ID: sea-139448
ABSTRACT
BACKGROUND:
Outer inflammatory protein A (OipA) is an outer membrane protein of Helicobacter pylori that is involved in inducing IL-8 and intracellular signaling. In this study, we have predicted exposure amino acid sequences of OipA for insertion in permissive sites of CstH subunit of Eschierchia coli CS3 pilli for bacterial surface display. MATERIALS ANDMETHODS:
Databases National Center for Biotechnology Institute and Protein Data Bank. Servers PHD, SABLE, GOR 4, SignalP3.0, TBBpred, PRODIV-TMHMM, TMRPres2D, CPH Models, PHYRE, GETAREA, VADAR, Pep state and pep window. Software Swiss PDB viewer and Discovery studio.RESULTS:
In silico prediction of exposure amino acid sequences of OipA led to detection of six sequences of amino acid, 76-87, 106-112, 170-182, 222-230, 242-258, and 278-290. These sequences inserted between amino acid sequences 66-67, 100-101, and 109-110 of CstH that were predicted by Eskandari et al. as permissive sites of CstH.CONCLUSION:
OipA has the ability to induce IL-8 from gastric epithelial cells and some papers are mentioned that this outer membrane protein involve to attachment and intracellular signaling. Receptor of OipA and adhesion motifs on this protein is unknown. Detection of exposure motifs aids to recognition of adhesion motifs and receptor of OipA on gastric epithelial cells. In this study, we have predicted exposure amino acid sequences for insert to subunit CstH of CS3 pilli E. coli for surface display.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Stomach
/
Bacterial Outer Membrane Proteins
/
Computer Simulation
/
User-Computer Interface
/
Helicobacter pylori
/
Amino Acid Sequence
/
Macrophage Inflammatory Proteins
/
Intracellular Signaling Peptides and Proteins
/
Epithelial Cells
/
Escherichia coli
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Hum Genet
Year:
2012
Type:
Article
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