Evaluation of the antimicrobial activity of various concentrations of Tulsi (Ocimum sanctum) extract against Streptococcus mutans: An in vitro study.
Article
in English
| IMSEAR
| ID: sea-139881
ABSTRACT
Aim:
To determine if Tulsi (Ocimum sanctum) extract has an antimicrobial activity against Streptococcus mutans and to determine which concentration of Tulsi (Ocimum sanctum) extract among the 15 concentrations investigated has the maximum antimicrobial activity. Setting andDesign:
Experimental design, in vitro study, Lab setting. Materials andMethods:
Ethanolic extract of Tulsi was prepared by the cold extraction method. The extract was then diluted with an inert solvent, dimethyl formamide, to obtain 15 different concentrations (0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 6%, 7% 8%, 9%, 10%) of the extract. 0.2% chlorhexidine was used as a positive control and dimethyl formamide was used as a negative control. The extract, along with the controls, was then subjected to microbiological investigation to determine which concentration among the 15 different concentrations of the extract gave a wider inhibition zone against Streptococcus mutans. The zones of inhibition were measured in millimeters using a vernier caliper.Results:
At the 4% concentration of Tulsi extract, a zone of inhibition of 22 mm was obtained. This was the widest zone of inhibition observed among all the 15 different concentrations of Tulsi that were investigated.Conclusion:
Tulsi extract demonstrated an antimicrobial property against Streptococcus mutans.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Solvents
/
Streptococcus mutans
/
Humans
/
Plant Extracts
/
Microbial Sensitivity Tests
/
Chlorhexidine
/
Ocimum
/
Dimethylformamide
/
Drug Evaluation, Preclinical
/
Anti-Infective Agents
Language:
English
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS