Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells.
Indian J Biochem Biophys
;
2012 Apr; 49(2): 86-91
Article
in English
| IMSEAR
| ID: sea-140222
ABSTRACT
In this study, the effect of cordycepin (3’-deoxyadenosine), a major component of Cordyceps militaris, an ingredient of traditional Chinese medicine was investigated for the first time on apoptotsis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells. Cordycepin significantly inhibited the proliferation of human neuroblastoma SK-N-BE(2)-C and human melanoma SK-MEL-2 cells with IC50 values of 120 mM and 80 mM, respectively. Cordycepin treatment at 120 mM and 80 mM, respectively, induced apoptosis in both cells and caused the increase of cell accumulation in a time-dependent manner at the apoptotic sub-G1 phase, as evidenced by the flow cytometry (FCM) and annexin V-fluorescein isothiocyanate (FITC) analyses. Western blot analysis revealed the induction of active caspase-3 and poly(ADP-ribose)polymerase (PARP) cleavage by cordycepin treatment. These results suggest that cordycepin is a potential candidate for cancer therapy of neuroblastoma and melanoma.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Cell Survival
/
Deoxyadenosines
/
Apoptosis
/
Cell Line, Tumor
/
Melanoma
/
Neuroblastoma
/
Antineoplastic Agents
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2012
Type:
Article
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