Comparison of the activities of amphotericin B, itraconazole, and voriconazole against clinical and environmental isolates of Aspergillus species.
Indian J Pathol Microbiol
;
2011 Jan-Mar 54(1): 112-116
Article
in English
| IMSEAR
| ID: sea-141928
ABSTRACT
Background:
Invasive fungal infections are a significant cause of morbidity and mortality in immunocompromised populations.Aims:
To evaluate the susceptibility pattern of our isolates against amphotericin B, itraconazole, and voriconazole and to compare the antifungal activities of these agents with each other against the Aspergillus species tested. Settings andDesign:
A prospective study was designed to include clinical and environmental isolates of Aspergillus species. Materials andMethods:
420 sputum samples, 70 bronchoalveolar lavage fluids, 160 oral washings, and 47 environmental samples were collected. Direct microscopy by potassium hydroxide and lactophenol cotton blue mounts followed by culture on Sabourad`s dextrose agar (SDA) was done. Susceptibility testing was performed by the broth microdilution technique as per Clinical Laboratory Standards Institute standards (M-38A). Additionally, all the isolates were also tested by the colorimetric microdilution technique using Alamar Blue dye. StatisticalAnalysis:
It was done by the Chi-square test and Z-test using SPSS statistical software version 12.0. Results andConclusion:
Twenty-seven isolates (47.3%) were recovered from patients with chronic bronchial asthma followed by fibrocavitary pulmonary tuberculosis in 9 (15.7%), allergic bronchopulmonary aspergillosis (ABPA) in 6 cases (10.5%), bronchiectasis in 3 (5.2%), bronchogenic carcinoma in 5 (8.7%) and those receiving radiotherapy for head and neck cancer 7 (12.2%). Thirteen environmental isolates were also included in the study. The most common isolate was A. fumigatus 28 (40%), followed by A. niger 22 (31%), A. flavus 13 (19%), and A. terreus 7(10%). All isolates were susceptible to amphotericin B, itraconazole, and voriconazole. Among the three agents tested, voriconazole exhibited lowest MICs (≤1 μg/ml) against all Aspergillus species.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pyrimidines
/
Aspergillosis
/
Aspergillus
/
Sputum
/
Triazoles
/
Humans
/
Bronchoalveolar Lavage Fluid
/
Microbial Sensitivity Tests
/
Amphotericin B
/
Itraconazole
Type of study:
Diagnostic study
/
Practice guideline
/
Observational study
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2011
Type:
Article
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