Clonality assessment of lymphoproliferative lesions using the polymerase chain reaction: An analysis of two methods.
Indian J Pathol Microbiol
;
2011 Apr-Jun 54(2): 330-334
Article
in English
| IMSEAR
| ID: sea-141994
ABSTRACT
Background:
Lymphoid malignancies are a heterogeneous group of disorders which may be difficult to differentiate from reactive proliferations even after immunohistochemistry. Polymerase chain reaction (PCR) is believed to be a good adjunct tool for diagnosis. Materials andMethods:
We examined 24 cases of neoplastic and non-neoplastic lymphoproliferative lesions in this study and evaluated the PCR as an additional tool in the confirmation of the diagnosis. Two different PCR methodologies were evaluated.Results:
In the evaluation of the T-cell PCR, it was seen that the correlation using both the commercial kits and the custom-synthesized primers was highly significant at a P value of <0.05. In the evaluation of the B-cell PCR, it was seen that the correlation using both the commercial kits and the custom-synthesized primers was not significant using either method (P > 0.05).Conclusions:
Both the methods showed an excellent concordance for T-cell γ gene rearrangements, However, the same was not seen in the B-cell receptor rearrangements. This may be because of the small sample size or the inability of consensus V primers to recognize complementary DNA sequences in all of the V segments.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Reagent Kits, Diagnostic
/
Humans
/
T-Lymphocytes
/
Polymerase Chain Reaction
/
Clone Cells
/
DNA Primers
/
Pathology, Molecular
/
Lymphoproliferative Disorders
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2011
Type:
Article
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