Crystal violet stain as a selective stain for the assessment of mitotic figures in oral epithelial dysplasia and oral squamous cell carcinoma.
Indian J Pathol Microbiol
;
2012 Jul-Sept 55(3): 283-287
Article
in English
| IMSEAR
| ID: sea-142254
ABSTRACT
Background:
Assessment of mitotic figures (MFs) is routinely practiced as prognostic indicator in oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC), but identification of MFs poses a problem in terms of staining characteristics.Aim:
To evaluate effectiveness of crystal violet stain for staining of MFs and its comparison with hematoxylin and eosin (H and E) stain. Materials andMethods:
Study sample includes archival tissues embedded in paraffin blocks diagnosed as OED (n = 30) and OSCC (n = 30). The control group comprised of tissue specimen from oral mucosa of healthy volunteers (n = 30). Two serial sections of each tissue specimen were stained separately with H and E stain and 1% crystal violet stain. The stained sections were observed under microscope for identification and counting of MFs. Data obtained was statistically analyzed by using the Man-Whitney U test.Results:
A significant increase in number of MFs was observed in OED and OSCC in comparison with normal oral mucosa. There was a highly significant increase in number of MFs in crystal violet stained tissue sections when compared with H and E stain. Metaphase is the most commonly observed phase of mitosis in crystal violet stain when compared with H and E stain for all three groups.Conclusion:
Crystal violet stain can be considered as selective stain for mitotic figures.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Staining and Labeling
/
Female
/
Humans
/
Male
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Adolescent
/
Adult
/
Young Adult
/
Gentian Violet
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2012
Type:
Article
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