Evaluation of a commercial Dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection.
Indian J Med Microbiol
;
2011 Jan-Mar; 29(1): 51-55
Article
in English
| IMSEAR
| ID: sea-143776
ABSTRACT
Purpose:
Dengue is one of the most serious mosquito-borne viral infections affecting tropical and subtropical countries in the world. Since there is no immunoprophylactic or specific antiviral therapy available, timely and rapid diagnosis plays a vital role in patient management and implementation of control measures. This paper evaluates a commercially available NS1 antigen capture ELISA vis-a-vis SD bioline Dengue NS1 antigen test for early detection of dengue virus. Materials andMethods:
To evaluate a commercial NS1 antigen detection kit vis-a-vis SD bioline Dengue NS1 antigen test, a total of 91 clinical samples were tested. Virological investigations with regard to dengue virus, viz. NS1 antigen capture ELISA (Panbio, Australia), SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were performed.Results:
Out of 91 samples, 24 (26%) were positive by NS1 antigen capture ELISA, 15 (16%) by SD bioline Dengue NS1 antigen test and 11(12%) positive by RT-PCR analysis. The RT-PCR-positive samples were further subjected to virus isolation and resulted in three isolates. The results of the Panbio NS1 antigen capture ELISA, SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were correlated among themselves.Conclusions:
The present study comprehensively established the utility of NS1 antigen ELISA in early diagnosis of dengue infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Reagent Kits, Diagnostic
/
Virology
/
Female
/
Humans
/
Male
/
Enzyme-Linked Immunosorbent Assay
/
Adolescent
/
Viral Nonstructural Proteins
/
Adult
/
Clinical Laboratory Techniques
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2011
Type:
Article
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