The role of a commercial enzyme immuno assay antigen detection system for diagnosis of C. trachomatis in genital swab samples.
Indian J Med Microbiol
;
2011 Oct-Dec; 29(4): 411-413
Article
in English
| IMSEAR
| ID: sea-143866
ABSTRACT
In the present pilot study, endocervical and urethral swabs collected from 100 patients attending sexually transmitted disease (STD) clinics and regional centre for STD in two referral hospitals in New Delhi were analyzed by enzyme immune assay (EIA), polymerase chain reaction (PCR) and direct fluorescent antibody (DFA) for detection of C. trachomatis. It was found that EIA could detect a very low number of cases (3/100) as against DFA (11/100) and PCR (9/100). Thus, in spite of the widespread availability, lower cost and ease of performance of the enzyme-linked-immunosorbent serologic assay, the present study highlights the need to employ sophisticated diagnostic tools like DFA and PCR for detection of Chlamydia trachomatis in STD patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Female
/
Humans
/
Male
/
Lymphogranuloma Venereum
/
Chlamydia trachomatis
/
Pilot Projects
/
Polymerase Chain Reaction
/
Immunoenzyme Techniques
/
Sensitivity and Specificity
/
Adolescent
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2011
Type:
Article
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