T-cell recognition of iron-regulated culture filtrate proteins of Mycobacterium tuberculosis in tuberculosis patients and endemic normal controls.
Indian J Med Microbiol
;
2012 Jul-Sept; 30(3): 323-331
Article
in English
| IMSEAR
| ID: sea-143978
ABSTRACT
Background:
Culture filtrate proteins (CFPs) of Mycobacterium tuberculosis are potential vaccine candidates.Objective:
The aim was to study the influence of iron levels on CFPs and assess the immuno-protective potential of defined antigenic fractions from high (8 μg Fe/mL) and low iron (0.02 μg Fe / mL) cultures of M. tuberculosis. Materials andMethods:
The CFPs of M. tuberculosis from high (CFP-high) and low (CFP-low) iron conditions were first compared to identify iron-regulated proteins and then fractionated to obtain ten antigen pools (CF-Ags H1- H5 and L1-L5) that were used to assess the immune response of TB patients and normal healthy controls.Results:
Iron limitation resulted in the up-regulation of two novel iron-regulated low-molecular-weight proteins Irp-1 (in CF-Ag L4) and Irp-2 (in CF-Ag L5) and repression of two ESAT proteins (identified with monoclonal antibody HYB 76.8). The median stimulation indices (SIs) against most of the CF-Ags were high in pulmonary TB patients. The CF-Ags L1 and L2 showed statistically significant SI (P values of 0.0027 and 0.0029 respectively); the % case recognition was high with these antigens as well as with L4 ( P = 0.0275). IFN-γ in response to these CF-Ags was significantly high in the endemic normals; maximal expression was seen with CF-Ag L5 (median value of 233 pg mL -1 ) that was higher than the corresponding H5 (140 pg mL -1 ) and H3 and L3 (205 and 206 pg mL -1 respectively).Conclusions:
CF-Ags L5, H3 and L3 showed immuno-protective potential in this geographical location.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Proteins
/
Tuberculosis
/
Female
/
Humans
/
Male
/
T-Lymphocytes
/
Adult
/
Iron
/
Mycobacterium tuberculosis
/
Antigens, Bacterial
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
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