Development and evaluation of reverse-transcription loop-mediated isothermal amplification for rapid detection of human immunodeficiency virus type 1.
Indian J Med Microbiol
;
2012 Oct-Dec; 30(4): 391-396
Article
in English
| IMSEAR
| ID: sea-143998
ABSTRACT
Purpose:
The objective of this study was to establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of human immunodeficiency virus type 1 (HIV-1). Materials andMethods:
The HIV-1 integrase gene region was selected because it was a conserved part of the HIV-1 genome. Six primers specific to eight regions of the HIV-1 integrase gene were designed. A total of 171 samples (18 HIV-1 confirmed positive samples and 153 serum specimens were collected in this study) were tested by RT-LAMP and reverse-transcription polymerase chain reaction (RT-PCR). After amplification in an isothermal water bath for 45 min, samples containing HIV-1 generated the expected ladder-like products while other viruses generated no product.Results:
The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with RT-PCR. The assay was significantly more sensitive than normal gel-based RT-PCR.Conclusion:
Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of HIV-1.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
RNA, Viral
/
Sensitivity and Specificity
/
HIV-1
/
Reverse Transcriptase Polymerase Chain Reaction
/
Nucleic Acid Amplification Techniques
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
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