Comparison of HIV-1 RNA level estimated with plasma and DBS samples: A pilot study from India (South).
Indian J Med Microbiol
;
2012 Oct-Dec; 30(4): 403-406
Article
in English
| IMSEAR
| ID: sea-144000
ABSTRACT
Purpose:
The use of dried blood spots (DBS) for HIV-1 viral load determination could greatly enhance the management of HIV infected individuals in resource-limited countries.Objective:
To compare the HIV-1 viral load values obtained between parallel collected plasma and DBS. Materials andMethods:
DBS and plasma samples were collected from 62 HIV-1 infected individuals and were used for determination of HIV-1 RNA concentrations using the Abbot real-time HIV-1 PCR.Result:
Mean of the log difference of viral load values between plasma and DBS was -0.41 log. DBS viral load values significantly correlated with plasma viral load (r = 0.9818, P < 0.0001).Conclusion:
These results suggest that DBS samples can be used as an alternative to plasma for the estimation of HIV-1 viral load if samples are appropriately stored.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Patients
/
Humans
/
RNA, Viral
/
Pilot Projects
/
HIV-1
/
Real-Time Polymerase Chain Reaction
/
India
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
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