Nanoactivator mediated modifications in thermostable amylase from Bacillus licheniformis.
Indian J Biochem Biophys
;
2012 Dec; 49(6): 468-471
Article
in English
| IMSEAR
| ID: sea-144089
ABSTRACT
Gram-positive rod-shaped thermophilic bacteria were isolated using samples collected from terrestrial natural thermal spring located at Unkeshwar (Longitude 78.22 degree East to 78.34 degree East, Latitude 19 degree 34' North to 19 degree 40' North), District Nanded, Maharashtra State, India. The isolates were then cultivated using selective media and identified using culture-dependent techniques. One prominent isolate (UN1) exhibited high temperature stability and remarkable amylase production and was identified as Bacillus licheniformis. Amylase production was carried out in starch media and the enzyme was partially purified and characterized for optimization of pH and temperature. Amylolytic activity of the enzyme was determined. Nanoactivator-mediated modifications were carried out to enhance amylolytic activity of the partially purified amylase. Three-fold increase in catalytic efficiency of amylase was obtained after modification.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacillus
/
Thermoplasmales
/
Nanostructures
/
Amylases
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2012
Type:
Article
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