Molecular analysis of oral squamous cell carcinoma: A tissue microarray study.
Indian J Cancer
;
2010 Apr-June; 47(2): 166-172
Article
in English
| IMSEAR
| ID: sea-144324
ABSTRACT
Background:
An intriguing aspect of Oral Squamous Cell Carcinomas (OSCC) is its behavioral disparity. Among patients who present with the similar clinicopathological features, some have a better prognosis than others. Identification of molecular alterations responsible for this may contribute to a greater understanding of tumor behavior. Tissue microarray (TMA) approach is a high throughput technology that enables analysis of multiple molecular targets simultaneously without causing any morphological alteration to tissue specimens. Aim andObjective:
To assess the tumor behavior based on the expression of p53, Bcl-2 and E-cadherin using TMA technology. Settings andDesign:
This was a case series analysis using tissue microarray technology. Materials andMethods:
Formalin-fixed Paraffin-embedded (FFPE) tissue blocks of histological proven cases of OSCC (n = 30) were retrieved from the department archives. Tissue microarray blocks were constructed; 4 ΅m thick sections were cut and immunostained for p53, Bcl-2 and E-cadherin. StastisticalAnalysis:
Mean (SD) was used to summarize age, frequencies with percentages was used to summarize categorical variable and Chi-square test was used to find association between histopathology evaluation and expression of Bcl-2, p53, E-cadherin. Results andConclusion:
Bcl-2 was the most frequently expressed biomarker. The expression of Bcl-2 was inversely related to the degree of differentiation (P = 0.005). The follow-up data showed that 63.6% of the cases that were positive for both Bcl-2 and E-cadherin were disease-free following treatment. Tissue microarray technology is a promising way to analyse multiple biomarkers simultaneously. The molecular data obtained from TMA will enhance diagnosis, provide better prognostication and will improve cancer treatment for individual patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Prognosis
/
Aged
/
Female
/
Humans
/
Male
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
Cadherins
/
Case-Control Studies
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Language:
English
Journal:
Indian J Cancer
Year:
2010
Type:
Article
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