Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and type 2 diabetes mellitus : A North Indian study.
Indian J Med Sci
;
2009 May; 63(5) 187-194
Article
in English
| IMSEAR
| ID: sea-145406
ABSTRACT
Background:
The vitamin D receptor (VDR) gene is a candidate gene for susceptibility to several diseases. Studies on association between VDR polymorphisms and risk of type 2 diabetes (T2DM) in different ethnic populations are yet inconclusive.Aims:
This study was conducted to evaluate association between VDR polymorphisms and genetic susceptibility to T2DM in the north Indian population. Settings andDesign:
One hundred clinically diagnosed T2DM patients and 160 healthy controls from the north Indian population were recruited for genetic association study. Materials andMethods:
Genomic DNA was extracted from blood and genotyped for the single nucleotide polymorphism SNPs of FokI (T/C) [rs2228570], BsmI (A/G) [rs1544410] and TaqI (C/T) [rs731236] by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Statistical Analysis Used Genotype distribution and allelic frequencies were compared between patients and controls. Mean values and odds ratios (ORs) with 95% confidence interval (CI) were calculated using SPSS software (version 15.0).Results:
The genotype distribution, allele and haplotype frequencies of VDR polymorphism did not differ significantly between patients and controls. Mean age and waist-hip ratio of patients were found to be associated with VDR polymorphism. Combination studies showed FFBbtt increased the risk of T2DM in north Indians.Conclusions:
Our data suggest that VDR gene polymorphism in combination of genotypes is associated with the risk of T2DM and thus requires further studies as a probable genetic risk marker for T2DM.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Polymerase Chain Reaction
/
Receptors, Calcitriol
/
Genetic Predisposition to Disease
/
Polymorphism, Single Nucleotide
/
Diabetes Mellitus, Type 2
/
Gene Frequency
/
Genotype
/
India
/
Middle Aged
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Med Sci
Year:
2009
Type:
Article
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