Mycobacterial ES-31 Serine Protease - A Biomarker for Mycobacterium tuberculosis - A preliminary Report.
Article
in English
| IMSEAR
| ID: sea-146786
ABSTRACT
There is a need for simple and reliable method to identify Mycobacterium tuberculosis from AFB smear positive cases. Utility of mycobacterial ES-31 serine protease as a marker to detect Mycobacterium tuberculosis bacilli was explored using Fluorescein isothiocyanate conjugated anti-ES-31 serine protease antibody. The presence of ES-31 serine protease in bacilli was indicated by green fluorescence on the cell surface. Green fluorescence was observed with M.tb. H37Ra bacilli and M.tb. H37Rv bacilli while no Fluorescence was observed with M. chelonae, Nocardia farcinicum as well as in E. coli showing the usefulness of ES-31 serine protease as a marker for identification of mycobacterium tubercle bacilli in cultures.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Year:
2009
Type:
Article
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