In planta detection of Xanthomonas axonopodis pv. commiphorae using fyuA and rpoD genes.
Indian J Exp Biol
;
2013 Jun; 51(6): 470-476
Article
in English
| IMSEAR
| ID: sea-147616
ABSTRACT
Guggal is tapped for extraction of medicinally important oleo–gum–resin (guggul) by inoculating the stem bark with natural gum suspension containing pathogenic bacterium Xanthomonas axonopodis pv. commiphorae (Xac). The tree dies in the process. In absence of any specific medium for isolation of Xac, it is difficult to assess spread of the pathogen within the plant. A PCR based molecular detection technique using fyuA and rpoD gene specific primers is described here. The primers amplified products only from Xac and not from host tissues or common saprophytes. The method was sensitive enough to produce positive signals for up to 4.4 bacterial cells or 2 pg target DNA per reaction mixture. However, PCR inhibitors present in plant tissues drastically reduced the limit of detection. A simple overnight incubation of surface sterilised plant tissues in nutrient medium was introduced to increase pathogen titre and to overcome this problem. This technique was successfully used to measure spread of Xac in plant tissues away from the site of inoculation. The pathogen showed preference for acropetal movement and did not spread to 7–8 cm below the site of inoculation till 15 days after inoculation. This suggests possibility to manage the disease through plant surgery.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Diseases
/
Resins, Plant
/
Triterpenes
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Gram-Negative Bacterial Infections
/
DNA Primers
/
Xanthomonas axonopodis
/
Limit of Detection
/
Genes, Bacterial
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
2013
Type:
Article
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