Improvised microinjection technique for mosquito vectors.
Article
in English
| IMSEAR
| ID: sea-147660
ABSTRACT
Background & objectives:
Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.Methods:
Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.Results:
The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. Interpretation &conclusions:
The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Year:
2012
Type:
Article
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