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Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
Indian J Exp Biol ; 2013 Oct; 51(10): 849-859
Article in English | IMSEAR | ID: sea-149391
ABSTRACT
Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 µM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 µM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 µM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Growth Regulators / Soybeans / Cell Differentiation / Germination / Cotyledon / Cell Proliferation / Desiccation / Plant Somatic Embryogenesis Techniques / Amino Acids / Acclimatization Language: English Journal: Indian J Exp Biol Year: 2013 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Growth Regulators / Soybeans / Cell Differentiation / Germination / Cotyledon / Cell Proliferation / Desiccation / Plant Somatic Embryogenesis Techniques / Amino Acids / Acclimatization Language: English Journal: Indian J Exp Biol Year: 2013 Type: Article