Analysis of cytotoxic T-lymphocyte-associated antigen-4 and MMP-9 genes’ methylation and their expression profiles with risk of non-alcoholic fatty liver disease.
Indian J Hum Genet
;
2013 Apr; 19(2): 144-149
Article
in English
| IMSEAR
| ID: sea-149420
ABSTRACT
OBJECTIVE:
To investigate the effect of promoter methylation of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and matrix metalloproteinases (MMPs) on the risk of non-alcoholic fatty liver disease (NAFLD). MATERIALS ANDMETHODS:
CTLA-4 and MMP-9 promoter methylation were investigated using a methylation-specific polymerase chain reaction (MS-PCR) in blood samples taken from 80 NAFLD individuals and 95 healthy controls. The expression levels of CTLA-4 and MMP-9 were also assessed in 10 blood and 9 liver tissues mRNA samples from NAFLD patients. These cases were compared to the blood (n = 10) samples of healthy controls with real-time quantitative reverse transcriptase PCR.RESULTS:
No significant relationship was found for methylation of CTLA-4 and MMP-9 between cases and controls. The relative expression of CTLA-4 and MMP-9 mRNA in NAFLD was not significantly different compared to healthy control samples.CONCLUSION:
For the first time, our outcomes indicate that the methylation status of CTLA-4 and MMP-9 genes has no significant function on the process of NAFLD.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Gene Expression
/
Risk Factors
/
Adult
/
Matrix Metalloproteinase 9
/
CTLA-4 Antigen
/
Non-alcoholic Fatty Liver Disease
/
Methylation
/
Middle Aged
Type of study:
Etiology study
/
Risk factors
Language:
English
Journal:
Indian J Hum Genet
Year:
2013
Type:
Article
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