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A novel combination of plant growth regulators for in vitro regeneration of complete plantlets of guar [Cyamopsis tetragonoloba (L.) Taub.].
Indian J Exp Biol ; 2013 Dec; 51(12): 1120-1124
Article in English | IMSEAR | ID: sea-150300
ABSTRACT
A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtained in the medium containing (mg/L) 2 IBA, 3 BA and 1 GA3. Shoot regeneration from the cotyledonary node explants was observed after 10-15 days. Regeneration of roots from these shoots occurred after 20 to 25 days. The regenerated plantlets showed successful acclimatization on transfer to soil. This protocol is expected to be helpful in carrying out various in vitro manipulations in this economically and industrially important legume.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Growth Regulators / Plant Shoots / Plant Roots / Plant Leaves / Cyamopsis / Plant Development / Gibberellins / Indoles / Kinetin Language: English Journal: Indian J Exp Biol Year: 2013 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Growth Regulators / Plant Shoots / Plant Roots / Plant Leaves / Cyamopsis / Plant Development / Gibberellins / Indoles / Kinetin Language: English Journal: Indian J Exp Biol Year: 2013 Type: Article